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. 2014 Nov 13;92(2):39. doi: 10.1095/biolreprod.113.112854

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FIG. 5 — Adm2, Calcrl, and Ramp mRNA in rat ovary. Top panel shows the 1.4% agarose gel photograph of RT-PCR product for Adm2, Calcrl, Ramp, Ram2, and Ramp3 mRNA amplified using rat gene-specific primers in ovaries collected from nonpregnant rats at diestrous (NP) and pregnant rats on Day 18 (D18) and Day 22 (D22) of gestation. Messenger RNA for Rn18s in the respective samples was also analyzed. Bottom panel shows the mean ± SEM of the ratios of specific mRNA levels to Rn18s. Asterisks indicate significant differences (*D18 vs. NP, **D22 vs. NP, and ***D22 vs D18; P < 0.005; n = 6).