Biomass composition of autotrophic (A) and heterotrophic (B) C. protothecoides and concentration composition of the measured intracellular metabolites of autotrophic (C) and heterotrophic (D) C. protothecoides. Biomass components of major cellular macromolecules were expressed based on mass fraction of dry cell weight. Metabolite concentrations were expressed in moles per liter, and percentages of total concentrations are in parentheses. Amino acids include Glu, Arg, Ala, Asp, Gln, Asn, Lys, Pro, Thr, Val, Gly, Ser, His, Leu, Ile, Met, Tyr, Trp, and Phe. Nucleotides include ATP, ADP, and AMP. Central carbon intermediates are γ-aminobutyric acid, (iso)citrate, malate, Glc/Fru-6-P, α-ketoglutarate, succinate, ribose-5-P, dihydroxyacetonphosphate, 6-phosphogluconate, phosphoenolpyruvate, fumarate, Hyp, and pyruvate. Redox cofactors are NAD+, NADH, NADP+, and NADPH, and coenzymes are acetyl-CoA and malonyl-CoA.