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Oxygen-reducing activities of recombinant GST and GST-FLV4 fusion protein. The reaction mixture contained 50 mm Tris-HCl (pH 7.4), 1 mm NADH, and the protein of interest (40 μg). Catalase (Cat; 200 units mL−1) and dithionite (Dt; 1 mm) were added as indicated. Purified GST protein served as a control.
