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. 2014 Dec 8;167(2):411–423. doi: 10.1104/pp.114.246959

Figure 6.

Figure 6.

Expression levels of nitrate and phosphate transporter genes in the wild type (WT) and the transgenic lines (OE1 and OE2). Wheat seedlings were grown for 20 d in the control nutrient solution, and then total RNA was isolated from the roots and used for gene expression analysis. A to I, Expressions levels of TaNRT1.1 (A), TaNRT2.1 (B), TaPHT1.1/1.9 (C), TaPHT1.2 (D), TaPHT1.3/4 (E), TaPHT1.5 (F), TaPHT1.6 (G), TaPHT1.7 (H), and TaPHT1.10 (I). J, Net NO3− flux rate. NO3− fluxes were measured by SIET with 0.2 mm NO3− in the measuring solution. Positive and negative flux values indicate efflux and influx, respectively. Data represent means ± se of at least three replicates. Asterisks indicate that differences between the wild type and the transgenic lines were significant at the 0.05 level.