Figure 2.

Identification of lacs4 and lacs9 mutant lines. A and B, Schematic gene structures of LACS4 (A) and LACS9 (B) depicting exons in dark gray and introns in light gray. For both genes, the independent T-DNA insertion sites are indicated. Arrows indicate positions of primers used for RT-PCR. C, RT-PCR analyses showing the absence of full-length transcripts of LACS4 in both lacs4 mutant alleles and LACS9 in both lacs9 mutant alleles. The two lacs4 lacs9 double-mutant lines are deficient in both transcripts. WT, Wild type.