Figure 4.

Overexpression of KFB39 and reduction of PAL protein level in Arabidopsis. A, Relative expression level of KFB39 in 5-DAG seedlings of the selected KFB39 overexpression lines. Its level in transgenic lines, after normalization to that of the UBIQUITIN10 control gene, was expressed relative to its level in an empty-vector control line. tt4, pal1, and c4h were the controls. WT, Wild type. B, Level of endogenous PAL protein in the KFB39 overexpression lines. The PAL proteins were detected with anti-PAL peptide antibody. The monoubiquitin immunoblot against the anti-ubiquitin antibody (Ub) served as a control for the amount of protein loading.