Alteration of the accumulation of anthocyanins, CTs, and lignin in KFB39-RNAi transgenic lines. A, Effect of KFB39 silencing on anthocyanin pigmentation. The top row shows the front view of 4-week-old control (left), kfb01/20/50 triple mutant (middle), and kfb01/20/50/KFB39-RNAi transgenic (right) rosettes; the bottom row shows the opposite view of control, mutant, and transgenic rosettes, illustrating the enhanced anthocyanin pigment in the petioles of the triple mutant and RNAi transgenic lines. Bar = 5 cm. B, Approximately 10-week-old kfb01/20/50/KFB39-RNAi transgenic plants showing pigmentation in the inflorescence stems. C, Change in the total lignin content in kfb01/20/50/KFB39-RNAi transgenic Arabidopsis. The acetyl-bromide lignin was measured in independent T2 lines. The data represent means ± sd with three biological replicates. CWR, Cell wall residue. D, Relative content of CTs in mature seeds of kfb01/20/50 triple mutant and kfb01/20/50/KFB39-RNAi transgenic plants. The data represent means ± sd from four biological repeats. WT, Wild type.