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. 2014 Dec 8;167(2):507–516. doi: 10.1104/pp.114.247460

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Figure 1. — WNK8 interacts with RACK1. A, Y2H assays. Yeasts expressing Gal4-AD and LexA-BD fusion proteins were grown on Synthetic Defined (SD)-Leu-Trp medium to validate efficient transformation or SD-Leu-Trp-His-Ura + 3-amino-1,2,4-triazole (3-AT) selection medium to test interactions of the indicated protein pairs. B, BiFC assay. Carboxyl terminal domain of yellow fluorescent protein (cYFP)-tagged WNK8 was expressed with amino-terminal domain of YFP (nYFP)-tagged RACK1A, RACK1B, or RACK1C in tobacco leaf epidermal cells. mCherry-mitochondrial marker was coinfected as the expression control. Complementation of split YFP and fluorescence of mCherry are shown. cYFP-tagged WNK8 expressed with nYFP-tagged P31 and nYFP-tagged RACK1A, RACK1B, and RACK1C expressed with cYFP-tagged P31 were used as negative controls.