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WNK8 phosphorylates RACK1. A, Recombinant GST or GST-tagged RACK1A, RACK1B, or RACK1C protein was incubated with [γ-32P]ATP and GST-WNK8 for 6 h at room temperature. Phosphorylated proteins were then separated with SDS-PAGE and detected with a phosphoimage analyzer. Bottom shows Coomassie Blue staining of the gel. B, Quantification of phosphorylation level of RACK1 proteins.
