Figure 5.

Genetic complementation of rack1a mutants. A, qRT-PCR analysis of RACK1A transcripts. Total RNAs were isolated from rosette leaves of 4-week-old plants. Amplification of ACTIN8 was used as a control. Shown are means of three replicates ± se. SA/TA, RACK1A^S122A/T162A; SD/TE, RACK1A^S122D/T162E. B, Seven-week-old transgenic plants. Plants were grown under a 10-h-light/14-h-dark photoperiod. C, Rosette leaf production, days to flowering, and Glc sensitivity. Shown are means of a minimum 10 plants ± se for rosette leaf production and flowering assays. The percentages of green seedlings were recorded 10 d after seeds had been transferred to germination conditions with 6% Glc. At 1% Glc, the percentages of green seedlings for all genotypes were 100%. Shown are means of three biological replicates ± se. *, Significant difference from rack1a-2 mutant, P < 0.05.