Skip to main content
. 2014 Dec 10;167(2):457–471. doi: 10.1104/pp.114.252379

Figure 1.

Figure 1.

CP29 phosphorylation kinetic in rice. A, Immunoblot of rice-isolated chloroplasts assayed with anti-CP29 polyclonal antibody. Before chloroplast isolation, leaves have been treated with HL (1,000 µmol photons m–2 s–1) for different time lengths (Tx indicates minutes of illumination after 6-h dark adaptation) and then dark incubated (Rx indicates minutes of dark incubation upon HL treatment). Tris-Gly SDS-PAGE 15% plus Urea 3M; 1 µg of total chlorophyll (Chl) per lane. B, Densitometrical analysis of immunoblot in A, determining the amount of P-CP29 with respect to the total amount of CP29 (totCP29). Average values have been obtained considering two independent biological replicates. C, Alkaline phosphatase (AlkPh) treatment on rice-isolated monomeric antenna complexes (obtained according to Betterle et al., 2009) from HL-treated samples. P-CP29 dephosphorylation has been evaluated as in A. One-quarter microgram of Chl per lane.