FIGURE 1.

Osteoblast-specific deletion of Men1 with OC-Cre. A, osteoblast-specific osteocalcin promoter driving expression of the Cre recombinase cDNA in the transgene. B, the target Men1^flox and the recombinant Δ3–8 Men1 alleles. Locations of the genotyping primers (A, C, and D) are indicated (arrows). Filled triangles, LoxP sites. C, genomic DNA from mouse tissues was used as a template for multiplex PCR amplification with primers A + C + D. Product A + D = 638 bp (recombinant Δ3–8 Men1 allele); product A + C = 239 bp (target Men1^flox allele). The Men1 gene deletion is specific for bone.