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. 2014 Dec 23;290(7):3910–3924. doi: 10.1074/jbc.M114.629899

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FIGURE 8. — Osteoblast-specific overexpression of menin. A, Col1a1-Menin-Tg transgene construct in which the osteoblast-specific 2.3-kb Col1a1 promoter drives expression of a FLAG-tagged human menin cDNA. β, rabbit β-globin intron; pA, SV40 polyadenylation signal. Arrows indicate positions of forward (F) and reverse (R) primers. B, tissue RNA (calvaria, cartilage, trachea, kidney, liver, and heart) was extracted, and RT-PCR was carried out. The Col1a1-Menin-Tg transgene was expressed specifically in bone in the founder shown. −RT, minus reverse transcriptase control. C, tissue protein (calvaria, femur, cartilage, and kidney) was extracted, and a Western blot was conducted with antibodies against FLAG (Col1a1-Menin-Tg transgene), menin (endogenous and exogenous menin), and β-tubulin (loading control). The transgene (menin) was expressed specifically in bone of the Col1a1-Menin-Tg mice.