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. 2014 Dec 23;290(7):4022–4037. doi: 10.1074/jbc.M114.617787

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FIGURE 3. — A–C, competition between Nb 70 and Nb 96 and between Nb 70 or Nb 96 and TNF for binding to immobilized hTNFR1. A, adjusted SPR sensorgrams of Nb 70 and/or Nb 96 for binding to hTNFR1. B and C, adjusted SPR competition sensorgrams for Nb 70 (B) and Nb 96 (C), and TNF for binding to hTNFR1. Nbs and TNF were applied in equal amounts at saturating concentrations (1 μm). Competition between two components occurs when the signal obtained by binding of a mixture of the two is lower than the sum of the signals obtained by each component individually. Nb 70 and Nb 96 do not compete with each other for binding to hTNFR1, nor do Nb 96 and TNF. This is in contrast to Nb 70, which binds the same epitope in hTNFR1 as TNF or overlaps it. The arrows indicate when the two components are applied together.