A–F, inhibition of inflammation in human inflamed colonic biopsies. Inflamed colonic biopsies were obtained from patients suffering from active Crohn disease. Incubation with TROS for 24 h reduced the initial inflammatory expression of IL6 (A), IL8 (B), and TNF (C), in comparison with Nb Alb-Ctrl-Ctrl. This was also reflected in the supernatant, resulting in reduced IL6 (D), IL8 (E), and TNF (F) secretion. Nb Alb-Ctrl-Ctrl, an irrelevant control Nanobody. qPCR data were normalized to stable housekeeping genes. The connected lines represent data from the same patient. Data were analyzed using a paired t test.