TABLE 3.
Antibodies used in these studies
| Antibody | Specificity | Source |
|---|---|---|
| Primary Abs | ||
| Actin (AC-18) | β-Actin | Sigma-Aldrich (Diegem, Belgium) |
| 9G3/P-Tyr18 | Tau; Tyr(P)18 | Medimabs (Montréal, Canada) |
| 12E8 | Tau; Ser(P)262/Ser(P)356 | Elan Pharmaceuticals (San Francisco, CA) |
| AD2 | Tau; Ser(P)396/Ser(P)404 | Sigma-Aldrich |
| AT8 | Tau; Ser(P)202/Thr(P)205 | Innogenetics (Ghent, Belgium) |
| AT180 | Tau; Thr(P)231 | Innogenetics |
| AT270 | Tau; Thr(P)181 | Innogenetics |
| P-S396 | Ser(P)396 | Merck-Millipore (Overijse, Belgium) |
| BT2 | Tau; aa 193–198 | Innogenetics |
| HT7 | Tau; aa 159–163 | Innogenetics |
| M19G | Tau; aa 1–19 | A. Delacourte (34) |
| MC1 | Tau; aa 5–15/312–322; conformation-dependent | Generous gift from P. Davies (59) |
| TauCter | Tau; aa 426–441 | A. Delacourte (34) |
| TAU1 | Tau; Ser198/Ser199/Ser202 | Chemicon (Temecula, CA) |
| TAU5 | Tau; aa 218–225 | BD Pharmingen (San Diego, CA) |
| TOC1 | Tau; aa 155–244/376–421; oligomer-specific | Generous gift from L. Binder (9) |
| ADx201 | Tau; aa 218–224 | Generated in this study |
| ADx201-bio | Tau; aa 218–224 | Generated in this study |
| ADx215 | Tau; aa 16–24 | Generated in this study |
| ADx215-bio | Tau; aa 16–24 | Generated in this study |
| ADx210 | Tau; conformational epitope | Generated in this study |
| ADx210-bio | Tau; conformational epitope | Generated in this study |
| Secondary Abs | ||
| GAM-HRP | Anti-mouse mAb | Thermo Fisher Scientific (Erembodegem, Belgium) |
| GAM-Alexa594 | Anti-mouse mAb | Abcam (Cambridge, UK) |
| RAMa-HRP | Anti-mouse mAb | Thermo Fisher Scientific |
a Rabbit anti-mouse.