FIGURE 3.

Knockdown of PHF8 effects H3K9me2 demethylation and cell cycle progression. A, 293T cells were transfected with a control (C), PHF8 siRNA #1, or PHF8 siRNA #2. The cell lysates were harvested for immunoblotting with the indicated antibodies. B, 293T cells were transfected with FLAG-PHF8 and FLAG-PHF8 S844A or siRNA resistance PHF8 and PHF8 S844A together with PHF8 siRNA. The cell lysates were harvested for immunoblotting with the indicated antibodies. C, HeLa cells were transfected with a control, CDK2 siRNA, or PHF8 siRNA. The cell lysates were subjected to immunoblot analysis with the indicated antibodies. D–F, HeLa cells were transfected with control or PHF8 siRNA. Then the cells were synchronized to early S phase, released for 3 and 6 h, and subjected to flow cytometry analysis (D). The percentages of S/G₂/M phase cells in different groups were calculated (E). The cell lysates were subjected to immunoblot analysis with the indicated antibodies (F). *, p < 0.01.