FIGURE 4.

Phosphorylation of PHF8 Ser-844 promotes H3K9me2 demethylation and S phase progression. A, 293T cells were transfected with the indicated plasmids. The cell lysates were harvested and subjected to immunoblotting analysis. B and C, HeLa cells were transfected with control, pCMV FLAG-PHF8, or pCMV FLAG-PHF8-S844A and pCMV GFP-H2B as a sorting marker. The cells were synchronized at early S phase by double thymidine treatment, released for 3 and 6 h, and subjected to flow cytometry analysis (B). The percentages of S/G₂/M phase cells in different groups were calculated (C). *, p < 0.05.