FIGURE 3.

Recombinant components of CRL5^SOCS2 assemble into the monomeric full-size protein complex. A, schematic representation of CRL5^SOCS2, which includes substrate receptor SOCS2, adaptor EloBC complex, scaffold Cul5, and RING domain protein Rbx2. B, SDS-polyacrylamide gel images of the purified protein complexes. C, table showing a comparison of theoretical protein molecular weights against values experimentally determined by SEC-MALS. D, SEC-MALS elution profiles for the individual components of CRL5^SOCS2 and their complexes, including full-size SOCS2-EloBC-Cul5-Rbx2 complex.