FIGURE 1.

GDE4 and GDE7 are novel members of the mammalian GP-PDE family. A, deduced amino acid sequences of the conserved catalytic regions of human GP-PDEs. Black boxes, identical amino acids; gray boxes, similar amino acids. Conserved amino acids are indicated by asterisks. B, schematic diagrams illustrating the primary structures of these three mouse GP-PDEs. Putative transmembrane regions are numbered from the N terminus. GDE represents the putative GDE domain. The sequence identity of the amino acids among the putative catalytic regions of GDE1, GDE4, and GDE7 is shown. C, phylogenetic tree of GP-PDE family members. The GDE domains of putative GP-PDEs from both prokaryotes and eukaryotes with mouse GP-PDEs (GDE1–7) are aligned. The phylogenetic tree indicates the closest evolutionary relationship between GDE4 and GDE7 proteins. ec, Escherichia coli; dm, Drosophila melanogaster; dr, Danio rerio; ce, Caenorhabditis elegans; sc, Saccharomyces cerevisiae; at, Arabidopsis thaliana.