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. 2014 Dec 20;290(7):4260–4271. doi: 10.1074/jbc.M114.614537

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FIGURE 2. — mRNA and protein expression of the mammalian GDE family genes in mouse tissues. A, total RNAs from mouse tissues were prepared and subjected to semiquantitative PCR analyses. GDE4 mRNA was mainly detected in the gastrointestinal tract, testis, and widely in brain regions. GDE7 mRNA was highly expressed in mouse kidney and stomach tissues. B, Western blot analysis of GDE4 and GDE7 in the gastrointestinal tract. GDE4 was distributed all over the gastrointestinal tract, whereas GDE7 was mainly detected in the mouse stomach among these tissues. C, in situ hybridization showing GDE4 mRNA in intestinal epithelial cells and in spermatids in testis sections. D, in situ hybridization showing GDE7 mRNA highly expressed in gastroesophageal epithelial cells.