FIGURE 1.
Experimental setup to observe Nap1-mediated nucleosome assembly with three-color single molecule FRET. A, 601 + 1–73 DNA construct labeled with Cy3 and Cy5.5 mixed with the (H3-H4)2 tetramer labeled with Atto647N. See the box in B for the identities of the colored diamond fluorophore labels. B, 601 + 16–63 DNA construct labeled with Cy3 and Cy5.5 mixed with a histone octamer. The histone H2A-H2B dimer is labeled with Atto647N. C, Left panel, positions of the dyad, +1st, and −73rd nucleotides. Right panel, positions of the dyad, +1st, and −63rd nucleotides (Protein Data Bank (PDB) ID: 3MVD).