Skip to main content
. 2014 Dec 30;290(7):4291–4303. doi: 10.1074/jbc.M114.619213

FIGURE 5.

FIGURE 5.

Kinetic scheme for Nap1-mediated nucleosome assembly according to our observation. TET and DI denote (H3-H4)2 tetramer and H2A-H2B dimer, respectively. The process of nucleosome assembly is divided into two paths according to the time point of H2A-H2B dimer incorporation relative to the tetrasome formation (i.e. complete DNA termini wrapping). Along the first path (path 1), Nap1 transfers a (H3-H4)2 tetramer to DNA, resulting in the formation of a stably positioned [DNA-TET] complex through an unstable [DNA-TET]* intermediate, followed by DNA termini wrapping to form a tetrasome. Incorporation of H2A-H2B dimers into the tetrasome completes nucleosome assembly. Along the second path (path 2), H2A-H2B dimers are incorporated to the [DNA-TET]* intermediate, resulting in a ternary [DNA-TET-DI] complex, followed by DNA termini wrapping to complete nucleosome assembly. Gray arrows are the processes that we could not observe with our setup due to the time limit of observation (20 min). The process times t1′, t1, t2, t3, t2′, and t3′ measured in Fig. 4 correspond to 1/k1′, 1/k1, 1/(k2 + k−2), 1/k3, and 1/(k3′ + k−3′), respectively.