FIGURE 8.
Blockade of GABAAR prevents water accumulation in the SEZ upon hypo-osmotic challenge. Quantitative analyses of the water uptake in the SEZ exposed to the indicated treatments ex vivo (A) or in vivo (B). A, after dissection, the tissue was incubated with or without bicuculline (Bic, 50 μm) for 30 min. Thereafter, tissue was treated with iso- (Ctr) or hypo-osmotic (Hypo) solution for a further 30 min, all at 37 °C. B, 30 min after i.v. microinjection with vehicle or bicuculline (30 pmol), iso- (Ctr) or hypo-osmotic solution was additionally microinjected as indicated. After a further 30 min, mice were sacrificed, and the SEZ was dissected and processed for the analysis of the water content. * indicates significantly different from vehicle + control group (ANOVA); n ≥ 3.