TABLE 1.
All primers used in the study
| Gene | Primer sequence | Accession no. | Note |
|---|---|---|---|
| per2 | AATCCCACGGAAATGAATCTC | ENSDART00000148788 | TALEN target fragment |
| ATCAGGCGACACAAGCTGAC | |||
| per2 | CGAATTCGCATGTCTAAAGACCTGGATTC | ENSDART00000148788 | cDNA cloning |
| TGGTACCTCAGGTGTCTGGACCGGGACAGC | |||
| rev-erbα | TGGATCCGCCACCATGACTTTACTGGGGCTCAAC | ENSDART00000126282 | cDNA cloning |
| GAAGCTTGGCATCAATGCGGAAAGACAG | |||
| rorαa | TGAATTCGCCACCATGTCAGAGTCACATGGATTC | ENSDART00000148537 | cDNA cloning |
| AGGATCCCCCGTCAACGGGCATCGACTG | |||
| rorαb | ACTCGAGGCCACCATGTACTTAATGATCACAGC | ENSDART00000019140 | cDNA cloning |
| AGGATCCGGCCTCCTGAGGCAGAATCTG | |||
| aanat2 promoter | AGGTACCAGTAGCCTAGAGCGCCATCTG | ENSDART00000018205 | Promoter cloning |
| CCTCGAGCTCCTCGCCTTTAACCCTCC | |||
| bmal1b promoter | AGGTACCACATGTTGGGGAAACTGGAG | ENSDART00000098259 | Promoter cloning |
| TCTCGAGATGAAGAAGGCAGGGAAGCAC | |||
| per1b | AGGAAGGCTGACAGATGATGAATG | ENSDART00000011082 | qRT-PCR |
| CCAGAGTGGGCTAAAGCGAAGTA | |||
| per2 | ACGAGGACAAGCCAGAGGAACG | ENSDART00000148788 | qRT-PCR |
| GCACTGGCTGGTGATGGAGA | |||
| per3 | GTTCTGGCGGAGTAATGGAG | ENSDART00000024304 | qRT-PCR |
| TGACGACGTTTTACTGGTGC | |||
| cry1aa | GACGCACAGCAGATAACAGGACG | ENSDART00000034401 | qRT-PCR |
| CAAAATGTAACAACTCGGGAAAAGG | |||
| cry1ba | TCGCCAAGTGCATAATTGGA | ENSDART00000100222 | qRT-PCR |
| GTGTGTCTCCCGAGGAAGGA | |||
| cry1bb | TCTACCAACAACTGTCCCGCTAC | ENSDART00000125347 | qRT-PCR |
| GCCATCCCATTTCCATTCCC | |||
| bmal1b | CCCTCTAGCTGTGGCTCAAG | ENSDART00000098259 | qRT-PCR |
| TCCCGCCATTGGACATCTTT | |||
| aanat2 | AGGACGCCATCAGTGTGTTT | ENSDART00000018205 | qRT-PCR |
| CTGGCCCAGGAAAACAAGTA | |||
| β-Actin | ACGAACGACCAACCTAAACTCT | ENSDART00000054987 | qRT-PCR |
| TTAGACAACTACCTCCCTTTGC | |||
| Luciferase | GTGTCCGATTCAGTCATGCC | Transgenic fish identification | |
| CTGGTTGTTTCTGTCAGGCC |