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. 2014 Dec 30;290(7):4398–4409. doi: 10.1074/jbc.M114.617035

FIGURE 6.

FIGURE 6.

Effects of JNK-inhibition on proliferation and apoptosis in isolated rat hepatocytes. A, after a culture period of 24 h, culture medium was replaced by medium containing BrdU. Then, hepatocytes were treated with insulin (100 nmol/liter), FFAs (each 50 μmol/liter) or a combination of both for another 48 h and analyzed for BrdU incorporation. When indicated, cells were pretreated (30 min) with SP600125 (100 μmol/liter) or L-JNKI-1 (5 μmol/liter). BrdU uptake by hepatocytes kept in control medium was set to 1. Statistical analyses of at least three independent experiments for each condition are shown. *, p < 0.05 denotes statistical significance between insulin plus FFA and after administration of JNK inhibitors. B, in another set of experiments, hepatocytes were stimulated with insulin (100 nmol/liter), FFAs (50 μmol/liter), or a combination of both for 18 h and the number of apoptotic cells was determined using TUNEL technique. Where indicated, cells were pretreated (30 min) with SP600125 (100 μmol/liter) or L-JNKI-1 (5 μmol/liter). Statistical analyses of at least three independent experiments for each condition are shown. *, p < 0.05 versus insulin plus FFA treatment.