TABLE 1.
Kinetic analysis of PSG1 interaction with the SLC and LAP using a 1:1 Langmuir binding model
The kinetic data shown in Fig. 2 for the interaction of PSG1-Fc and PSG1-His with the SLC and LAP of TGF-β1 were fit using a 1:1 Langmuir binding model for estimation of the association (ka) and dissociation (kd) rates and the dissociation constant (Kd = kd/ka).
| Kd | ka (mean ± S.D.) | kd (mean ± S.D.) | |
|---|---|---|---|
| μm | m−1 s−1 | s−1 | |
| SLC | |||
| PSG1-Fc | 2.56 | (3.10 ± 0.82) × 103 | (7.39 ± 0.83) × 10−3 |
| PSG1-His | 3.56 | (2.13 ± 0.86) × 103 | (7.56 ± 0.27) × 10−3 |
| LAP | |||
| PSG1-Fc | 2.05 | (2.32 ± 0.86) × 103 | (4.76 ± 0.42) × 10−3 |
| PSG1-His | 1.09 | (8.07 ± 0.31) × 103 | (8.81 ± 0.42) × 10−3 |