TABLE 3.

Transcriptional regulation by P1 length variants of the pbuE riboswitch in vitro

Mutant	Read-through transcription
	No ligand	1 mm 2-AP
	%
P1−4	2 ± 2	3 ± 3
P1−3	0 ± 2	0 ± 2
P1−2	5 ± 4	87 ± 3
P1−1	4 ± 4	80 ± 6
WT	2 ± 2	67 ± 4
P1+1	2 ± 2	64 ± 8
P1+2	6 ± 3	50 ± 3
P1+3	6 ± 3	90 ± 2
P1+4	47 ± 9	86 ± 6
P1+5	51 ± 6	92 ± 2
P1+6	91 ± 2	94 ± 2
P1+8	92 ± 2	91 ± 3
P1+10	91 ± 2	94 ± 2