Figure 1.

In vitro extracellular nerve recording. Application of H₂O₂ causes an increase in bladder afferent activity in young mice. (A) Representative trace showing changes in intravesical pressure and bladder afferent nerve activity following bath application of 0.003% and 0.03% H₂O₂. (B) Representation of changes in nerve firing in response to bath application of 0.003% and 0.03% H₂O₂ compared with baseline (N = 6; one-way ANOVA followed by Dunnet’s post hoc test, *P < 0.05 significant by Dunnet’s post hoc test). (C) Representation of changes in intravesical pressure following extravesical application of 0.003% and 0.03% H₂O₂ (N = 6; one-way ANOVA followed by Dunnet’s post hoc test, **P < 0.001, ***P < 0.0005, significant by Dunnet’s post hoc test). (D) Representation of changes in nerve firing in response to intravesical perfusion of 0.003% and 0.03% H₂O₂ compared with saline perfusion (N = 6; one-way ANOVA followed by Dunnet’s post hoc test, *P < 0.05 significant by Dunnet’s post hoc test).