Figure 4.

Rapamycin and calorie restriction do not alter thymic output. Spleen and superficial lymph nodes were harvested and analyzed for TRECs and Ag-specific CD8 T-cell precursors. (A) CD8 T-cell TRECs were quantified. Data shown are pooled from 2 independent harvests, each with n = 2–3 mice per group in each replicate. (B) CD8 T-cell precursors specific for the immunodominant WVN epitope NS4b were quantified. (C) Memory phenotype within the NS4b-specific CD8 T-cell precursors was measured. (B-C) Data are shown as mean ± SEM and are combined from 3 independent harvests, each with n = 2–3 mice/group at each harvest. (A) Statistical differences between O groups were calculated by nonparametric Kruskal–Wallis test with Dunn's multiple comparison test. Adults are shown only as a reference point and were not included in statistical analyses. (B, C) Statistical differences were calculated by 1-way ANOVA with Bonferroni's post-test