Figure 3.

GzmB deficiency protects against loss of dermal collagen density and fibronectin in UV-irradiated skin. (A) Dorsal skin sections were stained with picrosirius red and visualized under polarized light. Quantification of collagen was achieved by color segmentation and intensity analysis of stained sections. Scale bars = 100 μm. (B) Second-harmonic generation signals of collagen from intact ex vivo skin samples were quantified as a measure of collagen density. Scale bars = 20 μm (*P < 0.05; **P < 0.01; ***P < 0.001 Tukey's multiple comparison). (C) Fibronectin was assessed in tissue homogenates via Western blot. Results are presented as a percentage of wild-type nonirradiated control (WT-C) (mean ± SEM; *P < 0.05 Dunnett's multiple comparison).