Table 1.
Dual RNAi effects on mean aggregate numbers per worm
| RNAi-1 | RNAi-2 | Mean count | SEM | KD, % of control | Two-tailed t-test | Predicted KD% (ifindependent) |
|---|---|---|---|---|---|---|
| FV (empty vector) | FV | 64.5 | 1.1 | – | – | – |
| age-1 | FV | 52.5 | 2.2 | 81 | 1.5E−05 | – |
| daf-16 | FV | 49.1 | 1.6 | 76 | 4.0E−09 | – |
| hsf-1 | FV | 57.0 | 1.4 | 88 | 1.6E−04 | – |
| pha-4 | FV | 53.8 | 1.2 | 83 | 1.6E−07 | – |
| cram-1 | FV | 51.4 | 1.0 | 80 | 1.3E−09 | – |
| age-1 | cram-1 | 33.7 | 1.3 | 52 | 5.7E−18 | 0.81 × 0.80 = 65% |
| daf-16 | cram-1 | 40.1 | 1.9 | 62 | 9.9E−13 | 0.76 × 0.80 = 61% |
| hsf-1 | cram-1 | 40.7 | 1.0 | 63 | 1.1E−17 | 0.88 × 0.80 = 71% |
| pha-4 | cram-1 | 38.9 | 1.6 | 60 | 1.5E−14 | 0.83 × 0.80 = 67% |
Dual RNAi AM141 worms were assessed at 4 days posthatch (dPH), 14–19 fields per group with 1–4 worms per field. Aggregate counts for fields with multiple worms were divided by N and treated as single data points. Significance was assessed by two-tailed, homoscedastic t-tests, for differences between each indicated treatment group and the feeding vector control (FV/FV, no RNAi). Predicted knockdown percent for dual RNAi treatments was calculated by multiplying the percent of control for each RNAi used alone.