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. 2014 Aug 19;13(6):1001–1011. doi: 10.1111/acel.12263

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© 2014 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Triglyceride, diacyl-glycerol, and ceramide content in tibialis anterior muscle of young and old rats fed a low-fat or a high-fat diet for 10 weeks. Triglyceride (A), diacyl-glycerol (B), and ceramide (C) concentrations in tibialis anterior muscle were analyzed by gas-liquid chromatography after total lipid extraction. Ceramide, diacyl-glycerol (DAG) and triglyceride (TG) concentrations are expressed as nmoles/mg proteins. Values ± SEM for five animals per group. *P < 0.05 age effect (same diet), ^$P < 0.05 diet effect (same age). LFD, low-fat diet; HFD, high-fat diet.