Figure 6.

CD39^high cells in amyloid precursor proteins (APP)/PS1 Tg mice have increased expression of CD11b and CD45. (A) Flow cytometry profiles showing CD39 expression by CD11b⁺CD45⁺ gated cells in 21-month-old WT and APP/PS1 Tg mice. Cell populations were defined based on fluorescence levels in isotype-stained control (left panel) and identified throughout the figure as CD39⁻ (black), CD39⁺ (red), and CD39^high (purple). Quantification of FACS data showing %CD39⁺ among CD11b⁺CD45⁺ cells (B) and CD39 mean fluorescent intensity (MFI) (C) in WT and APP/PS1 Tg mice. (D) Percent CD39^high cells among CD39⁺ cells. (E) Numbers of CD39⁺ and CD39^high CD11b⁺CD45⁺ cells in WT and APP/PS1 Tg mice. (F) CD45/CD11b plots from WT and APP/PS1 Tg mice at 4 and 21 months of age, with CD39 subpopulations identified by multicolor gating, as defined above. CD11b (G) and CD45 (H) MFIs among CD39⁺ cells in WT and APP/PS1 Tg mice. *P < 0.05, **P < 0.01.