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Mitochondrial biogenesis-related gene expression levels in apelin KO mice. (a–e) Real-time reverse transcriptase (RT)-PCR analysis of myocardial expression of PGC-1α, NRF-1, TFAM, CPT1 and Sirt3 in apelin KO or WT mice. (f) Western blot analysis and densitometric evaluation of Sirt3 level in apelin KO (n=5) or WT (n=5) mice. Results are means±s.e.m. *P<0.05; **P<0.01 vs WT mice.
