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. 2014 Sep 10;8(2):414–428. doi: 10.1038/mi.2014.79

Figure 4.

Figure 4

CD83 engages in homotypic binding at the cell surface. (a–d) CD83 binds to cells expressing CD83. (a, b) CD83 expression. (a) CD83 expressed on the surface of stably transfected CHO-hCD83 cells (black line) but not on control CHO cells (dashed line). (b) CD83 expressed on MUTZ-3-derived mature dendritic cell (mDC; black line), but at very low levels on immature DC (iDC; gray line). Solid histograms represent isotype controls. (c, d) CD83.Fc binds to cells expressing CD83 on surface. (c) Binding of CD83.Fc to CHO-hCD83 (black line) but not to control CHO cells (dashed line). (d) CD83.Fc binds to surface of mDC (black), but not iDC (gray). Solid histograms in c and d represent binding with control hIgG1.Fc protein. (e) Anti-CD83 antibody blocks binding to CHO-hCD83. CD83.Fc binds to CHO-hCD83 cells (black line); binding is blocked by αCD83 (dashed line), but not isotype control (gray line). Solid histograms in e and h represent binding with control hIgG1.Fc protein (f) CD83 siRNA efficacy. Taqman analysis of total RNA from MUTZ-3 mDCs treated with non-targeting control (siNTC) siRNA or siRNA specific to CD83 (siCD83), normalized to 18S. (g) CD83 is expressed on surface of mDC treated with siNTC (black line) but not on iDCs or mDCs treated with siCD83 (dashed line). (h) CD83.Fc binds to mDC; binding is abrogated by siCD83.