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Schematic illustration of the strategy for the “on-off” type of fluorescent nanobiosensors for AChE inhibitors. (a) Fabrication of AChE-AuNP conjugate via EDC/NHS coupling chemistry; (b) Metal enhanced fluorescence of DDAO bound with the active sites of AChE via distance regulation between the fluorophore and AuNP; (c) The fluorescence reduction via distance modulation due to AChE inhibitor binding. Reprinted with permission from [63]. Copyright 2012 American Chemical Society.
