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. 2015 Feb 15;128(4):683–694. doi: 10.1242/jcs.157834

Fig. 5.

Fig. 5.

ActA effects mononuclear and multinucleated cell apoptosis. (A) mBMMs were grown in the presence of MCSF and RANKL for 5 days before a 24-hour treatment with MCSF alone (20 ng/ml) or RANKL (50 ng/ml), ActA (50 ng/ml), or RANKL plus ActA to assess apoptosis. Left panels, DAPI (blue) and cleaved caspase-3 (green). Right panels show the overlay of DAPI, cleaved caspase-3, and Texas-Red-conjugated Phalloidin (red). Closed arrowheads, multinucleated cells positive for cleaved caspase-3; open arrowheads, F-actin phalloidin-stained ring; closed arrows, mononucleated cells positive for cleaved caspase-3. Scale bars: 100 µm. (B) Total number of multinucleated cells positive for cleaved caspase-3 after 24 hours. (C) Total number of mononucleated apoptotic cells positive for cleaved caspase-3 cells after 24 hours. Each bar represents the mean±s.d. from triplicate wells from two independent experiments. Different letters indicate significantly different groups (P<0.05).