Fig. 2.

Localisation of peripheral Hrs to SNX15 but not APPL1 endosomes. (A) HeLaM cells were co-stained for APPL1 and Hrs, and were examined by wide-field microscopy. (B) HeLaM cells were stained for clathrin and Hrs and imaged by TIRF microscopy. Arrows show examples of structures containing both Hrs and clathrin. (C) HeLaM cells were transfected with GFP–SNX15, fixed and stained for EEA1 and Hrs and examined by wide-field microscopy. The insets show a ×3 magnification of the indicated areas. Values show net percentages (i.e. scrambled values subtracted) of particles labelled by marker 1 that are also labelled by marker 2. Scale bars: 10 µm.