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EGF labels distinct Hrs/SNX15-positive and APPL1-positive endosomes. Untransfected RPE cells (A) or RPE cells transfected with GFP–SNX15 (B) were serum starved and then pulse-chased with fluorescent EGF for the indicated total times and labelled for immunofluorescence. Cell outlines are shown in white. The insets show a ×3 magnification of the indicated areas. Scale bars: 10 µm.
