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Localisation of ESCRT-I to early endosomes. RPE cells were serum starved and pulse-chased with fluorescent EGF for the indicated total times, permeabilised with saponin and labelled for VPS28 and Hrs (A) or EEA1 (B). Small arrows show examples of endosomes containing EGF and Hrs or EEA1 but lacking VPS28 labelling. Large arrows highlight example endosomes containing all three markers. Cell outlines are shown in white. Scale bars: 10 µm.
