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. 2015 Feb 15;128(4):755–767. doi: 10.1242/jcs.161786

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Localisation of ESCRT-III to early endosomes. RPE cells were serum starved and pulse-chased with fluorescent EGF for the indicated total times, permeabilised with saponin and labelled for CHMP4B and Hrs (A) or EEA1 (B). Arrows with open arrowheads show examples of CHMP4B in unstimulated cells colocalising with Hrs or EEA1. Small arrows show examples of endosomes containing EGF and Hrs or EEA1 but lacking CHMP4B labelling. Large arrows highlight example endosomes containing all three markers. Scale bar: 10 µm.

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