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ESCRT-0 is required for EGF transit to EEA1 endosomes. HeLaM cells transfected with control siRNA or Hrs siRNA oligo 1 were pulse-chased with fluorescent EGF for the indicated total times, fixed for immunofluorescence and labelled with anti-EEA1. Cell outlines are shown in white. k/d, knockdown. Scale bars: 10 µm.
