Figure 2.

Triple-stained example section of the SOC region of a young adult gerbil. The section corresponds to the reference series at rc-level 6 (E). In (A,B,C) MAP2 stain, ChAT stain and CSPG stain are depicted alone with delineated SOC nuclei. Overlays of ChAT (Alexa Fluor 488, green) and MAP2 (Alexa Fluor 647, red) are shown in (D) and ChAT and CSPG (Cy 3, 570; red) in (F). ChAT positive cells were easily detected in the ChAT stain alone as well as in overlay with both overview stains (MAP and CSPG). Scale bar in (A–F): 500 µm.