Figure 4.

Selective interaction of C. aurantium extract with glutamate receptors In vitro. Synaptic membranes were incubated with C. aurantium extract at 1.4 mg/mL in the presence of 1 mM of agonists for iGluR's o mGluR's and 20 nM [³H]Glutamate. For these experiments, nonspecific binding was 22% of total binding (represented with a dotted line). All treatments significantly reduced [³H]Glu binding, when compared with Total binding. (A) C. aurantium 1.4 mg/mL reduced ligand binding in the presence of ionotropic glutamate receptor agonist for NMDA, AMPA, and KA receptors compared to the agonist alone. (B) C. aurantium 1.4 mg/mL reduced ligand binding in the presence of metabotropic glutamate receptor agonist for mGluR II (LCCG-I, DCG-IV) and mGluR III (L-AP4) when compared to the agonist alone. Results are shown as percentage of total binding ± SEM of three experiments. ⁺⁺ vs. Agonist P < 0.01; ⁺⁺⁺⁺P < 0.0001. ^## vs. CA1.4 mg/ml P < 0.01; ^###P < 0.001.