Figure 5.

Selective In vivo interaction of C. aurantium extract with iGluR. Fish were allowed 1 h absorption of various concentrations of selective iGluR antagonist D-AP5 (NMDAR), NBQX (AMPA), UBP 301 (KAR) followed by 1 h absorption on C. aurantium extract at 28 mg/mL prior exposure to PTZ 3 mg/mL. (A) D-AP5 2.7 and 6 μM prior exposures to C. aurantium extract significantly reduced seizure latency when compared to C. aurantium alone. (B) Exposure to NBQX prior exposures to C. aurantium extract showed a tendency to reduce seizure latency when compared to C. aurantium alone. (C) Exposure to UBP 301 prior exposures to C. aurantium extract had no significant effect on seizure latency when compared to C. aurantium alone. Results are shown as average ± SEM of at least three experiments, n > 12. ^**** vs. Naive P < 0.0001, ^## vs. C. aurantium 28 mg/mL P < 0.01.