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. 2015 Feb 13;6:52. doi: 10.3389/fmicb.2015.00052

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Copyright © 2015 Cook, Crooks, Hussain, O’Brien, Braitch, Kareem, Constantinescu, Robinson and Gran.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Proliferation of splenocytes in response to stimulation by MOG_35-55 peptide. Mice were infected with H. pylori (Hp/EAE), or given a placebo dose (Broth/EAE). After 3 weeks, EAE induction treatment with MOG_35-55 peptide was administered. Splenocytes were isolated from mice after a further 3 weeks. Splenocytes were stimulated with MOG_35-55 peptide and incorporation of [³H]thymidine was used to measure the proliferative response in counts per minute (CPM). The median CPMs following stimulation with anti-CD3/28 antibodies were 118946 and 57633 for the Broth/EAE and Hp/EAE groups, respectively. Bars depict the mean CPM for mice in each group; error bars represent SD. p-values were calculated using Mann–Whitney U-tests.