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. 2015 Feb 13;6:52. doi: 10.3389/fmicb.2015.00052

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Copyright © 2015 Cook, Crooks, Hussain, O’Brien, Braitch, Kareem, Constantinescu, Robinson and Gran.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometry analysis of splenocytes from infected and uninfected mice with EAE. Groups of six mice were orally inoculated with H. pylori (Hp/EAE) or given Brucella broth as a placebo (Broth/EAE). After 3 weeks EAE was induced in all mice. Spleens were disrupted, stained using fluorochrome-conjugated antibodies and analyzed using flow cytometry. Graphs show the proportion of CD4⁺ events which expressed the transcription factors T-bet, RORγt, and Foxp3 (A), and the proportion of CD4⁺ events which expressed the cytokines IFNγ and IL-17 (B). Medians are shown with a horizontal line. p-values were calculated using Mann–Whitney U-tests.