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. Author manuscript; available in PMC: 2015 Feb 13.
Published in final edited form as: Prog Mol Biol Transl Sci. 2010;95:237–298. doi: 10.1016/B978-0-12-385071-3.00009-5

Figure 1. Schematic representation of the Akt kinase reporter (BAR).

Figure 1

A, The chimeric Akt kinase reporter is a fusion of the N-Luc and C-Luc luciferase protein linked to an Akt peptide domain containing the Akt substrate sequence and a yeast FHA2 phospho-Ser/Thr binding domain. B, Phosphorylation of the Akt substrate peptide in the reporter results in interaction with the FHA2 phospho-peptide binding domain leading to steric constraints on the N-Luc and C-Luc. Inhibition of Akt kinase activity results in decreased binding of phospho-peptide and the binding domain which enables reconstitution of luciferase and restored bioluminescence.