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. Author manuscript; available in PMC: 2015 Feb 13.
Published in final edited form as: Prog Mol Biol Transl Sci. 2010;95:237–298. doi: 10.1016/B978-0-12-385071-3.00009-5

Figure 10. Schematic of the transgenic apoptosis reporter construct.

Figure 10

A, the transgene depicted contains the ER-LACZ-ER coding sequence (see Figure 7) under the transcriptional control of the keratin 5 (KRT5) promoter. B & C, Immunohistochemical analysis of formalin-fixed, paraffin-embedded dorsal skin sections from nontransgenic (control) (B) and transgenic (C) mice (ER-LACZ-ER transgenic). Presence of β-galactosidase protein and KRT5 was detected using the appropriate antisera. DAPI (4,6-diamidino-2-phenylindole) staining was used to identify nuclei. In control animals, KRT5 staining but not b-galactosidase staining was observed, whereas in transgenic animals, KRT5 staining and β-galactosidase staining was detected in similar populations within the section. Bar = 40 μm. (From [48], with permission).